Size  100ul
Cat code  220024
Species Cross-reactivity  H,R
Key Application  WB
Host  Mouse
Clone Number  1E5-D6-G7
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  44kDa
Sensitivity  The antibody detects endogenous level of total MAPK3 protein.
Immunogen  Purified recombinant fragment of human MAPK3 expressed in E. Coli.
UniProt Number  P27361
GeneBank ID  NM_002746.2
Subcellular location  Cytoplasm. Nucleus. Note: Autophosphorylation at Thr-207 promotes nuclear localization.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  ERK 1 ; ERK ; ERK-1 ; ERK1 ; ERT 2 ; ERT2 ; Extracellular Signal Regulated Kinase 1 ; Extracellular signal related kinase 1 ; Extracellular signal-regulated kinase 1 ; HGNC6877 ; HS44KDAP ; HUMKER1A ; Insulin Stimulated MAP2 Kinase ; Insulin-stimulated MAP2 kinase ; MAP kinase 1 ; MAP kinase 3 ; MAP Kinase ; MAP kinase isoform p44 ; MAPK 1 ; MAPK 3 ; MAPK ; MAPK1 ; Mapk3 ; MGC20180 ; Microtubule Associated Protein 2 Kinase ; Microtubule-associated protein 2 kinase ; Mitogen Activated Protein Kinase 3 ; Mitogen-activated protein kinase 1 ; Mitogen-activated protein kinase 3 ; MK03_HUMAN ; OTTHUMP00000174538 ; OTTHUMP00000174541 ; p44 ERK1 ; p44 MAPK ; p44-ERK1 ; p44-MAPK ; P44ERK1 ; P44MAPK ; PRKM 3 ; PRKM3 ; Protein Kinase Mitogen Activated 3 ;
Gene_symbol  ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Summary  The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Application Images :  
Western blot detection of MAPK3 antibody in PC12, K562 and Hela cell lysates using MAPK3 antibody(dilution 1:500).Predicted band size:44KDa.Observed band size:44KDa.