Size  100ug
Cat code  200744
Species Cross-reactivity  H,M,R
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  null
Isotype  IgG1
Molecular Weight  49kDa
Sensitivity  This antibody detects endogenous levels of HDAC3 and does not cross-react with related proteins.
Immunogen  Purified recombinant human HDAC3 protein fragments expressed in E.coli.
UniProt Number  O15379
GeneBank ID  NM_003883.3
Subcellular location  Nucleus
Formulation  ascites
Altername  RPD3-2
SMAP45
Gene_symbol  HD3; RPD3; RPD3-2 
Summary  Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
UniPort summary  Function  Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation
Application Images :  
Western blot detection of HDAC3 in THP-1,LNCAP,MCF7,PC12,293,Jurkat,3T3&Hela cell lysates using HDAC3 antibody (1:1000 diluted).
Predicted band size:49KDa.
Observed band size:49KDa