Size  100ug
Cat code  200312
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  296kDa
Sensitivity  This antibody detects endogenous levels NSD1 and does not cross-react with related proteins.
Immunogen  Purified recombinant human NSD1 protein fragments expressed in E.coli.
UniProt Number  Q96L73
GeneBank ID  NM_022455
Subcellular location  Cytoplasm. Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  STO; KMT3B; SOTOS; ARA267; FLJ10684; FLJ22263; FLJ44628; DKFZp666C163
Gene_symbol  HSF2; HSTF2
Summary  This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene.
UniPort summary  Function  DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Application Images :  
Western blot detection of NSD1 in HepG2-LPS&HepG2 cell lysates and  using NSD1 antibody (1:1000 diluted). 
Predicted band size: 297KDa 
Observed band size: 300KDa