Size  100ug
Cat code  200314
Species Cross-reactivity  H
Key Application  WB,IHC,ICC,FC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  260kDa
Sensitivity  This antibody detects endogenous levels CHD3 and does not cross-react with related proteins.
Immunogen  Purified recombinant human CHD3 protein fragments expressed in E.coli.
UniProt Number  Q12873
GeneBank ID  NM_001005271
Subcellular location  Nucleus. Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  ZFH; Mi-2a; Mi2-ALPHA
Gene_symbol  ZFH; Mi-2a; Mi2-ALPHA
Summary  This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
UniPort summary  Function  Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Application Images :  
Western blot detection of CHD3 antibody in Y79&Raji cell lysates using CHD3 antibody (1:1000 diluted).
Predicted band size: 226KDa
Observed band size: 260KDa
Immunocytochemistry of HeLa cells using anti- CHD3 (C-terminus) antibody diluted 1:150.
IHC of paraffin-embedded human colon using anti- CHD3 (C-terminus) diluted 1/500-1/1000.
Flow Cytometry analysis of K562 cells stained with CHD3 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.