Size  100ug
Cat code  200955
Species Cross-reactivity  H,M,R
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  42kDa
Sensitivity  This antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.
Immunogen  Purified recombinant human WDR77 protein fragments expressed in E.coli.
UniProt Number  Q9BQA1
GeneBank ID  NM_024102
Subcellular location  Nucleus.Cytoplasm.
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername  2610312E17Rik;Androgen receptor cofactor p44;C79984;HKMT1069;MEP 50;MEP-50;MEP50;MEP50_HUMAN;Methylosome protein 50;MGC2722;Nbla10071;p44;p44/Mep50;RGD1310479;RP11 552M11.3;WD repeat containing protein 77;WD repeat domain 77;WD repeat-containing protein 77;WDR77.
Gene_symbol  p44;MEP50;MEP-50;HKMT1069;Nbla10071;p44/Mep50;RP11-552M11.3.
Summary  WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061).This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al.,2002 [PubMed 11756452]).[supplied by OMIM,Mar 2008]
UniPort summary  Function  Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Application Images :  
Western blot detection of WDR77 antibody in C6,3T3 and K562 cell lysates using WDR77 antibody (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100，next using anti-WDR77 antibody (dilution 1:100).