Size  100ug
Cat code  200054
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  220kDa
Sensitivity  This antibody detects endogenous levels of KDM5C and does not corss-react with related proteins
Immunogen  Purified recombinant human KDM5C (C-terminus) protein fragments expressed in E.coli
UniProt Number  P41229
GeneBank ID  NM_004187.3
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Gene_symbol  MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Summary  This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternative splicing results in multiple transcript variants.
UniPort summary  Function  Histone demethylase that specifically demethylates ´Lys-4´ of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 ´Lys-9´, H3 ´Lys-27´, H3 ´Lys-36´, H3 ´Lys-79´ or H4 ´Lys-20´. Demethylates trimethylated and dimethylated but not monomethylated H3 ´Lys-4´. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Application Images :  
Western blot detection of KDM5C(C-terminus) antibody in Hela&293 cell lysates using KDM5C(C-terminus) antibody (1:1000 diluted).
Predicted band size: 176KDa
Observed band size: 220KDa
Immunocytochemistry of HeLa cells using anti- KDM5C (C-terminus) antibody diluted 1:150.