Size  100ug
Cat code  200922
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  133kDa
Sensitivity  This antibody detects endogenous levels of PROM1 and does not cross-react with related proteins.
Immunogen  Purified recombinant human PROM1 protein fragments expressed in E.coli.
UniProt Number  O43490
GeneBank ID  NM_006017
Subcellular location  Cell projection ? cilium ? photoreceptor outer segment.Isoform 1: Apical cell membrane;Multi-pass membrane protein By similarity.Cell projection ? microvillus membrane;Multi-pass membrane protein.
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  null
Gene_symbol  RP41;AC133;CD133;MCDR2;STGD4;CORD12;PROML1;MSTP061.
Summary  This gene encodes a pentaspan transmembrane glycoprotein.The protein localizes to membrane protrusions and is often expressed on adult stem cells,where it is thought to function in maintaining stem cell properties by suppressing differentiation.Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease.Expression of this gene is also associated with several types of cancer.This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner.Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq,Mar 2009]
UniPort summary  Function  Binds cholesterol in cholesterol-containing plasma membrane microdomains.Proposed to play a role in apical plasma membrane organization of epithelial cells.During early retinal development acts as a key regulator of disk morphogenesis.Involved in regulation of MAPK and Akt signaling pathways.In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Application Images :  
Western blot detection of PROM1 antibody in CaCo2 cell lysate using PROM1 antibody (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa
Western blot detection of PROM1 expression in CHO-k1 cells non-transfected (A) or transfected (B) with PROM1 and using PROM1 antibody (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa.