Size  100ug
Cat code  200068-8F10
Species Cross-reactivity  H,M,Mk,G,R,Hm,S.cerevisiae,Arabidopsis,pichia pastoris,Rb
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  45kDa
Sensitivity  This antibody detects endogenous levels of beta-actin and does not cross-react with related proteins.
Immunogen  Purified recombinant human beta-actin protein fragments expressed in E.coli.
UniProt Number  P60709
GeneBank ID  NM_001101
Subcellular location  Cytoplasm?cytoskeleton
Formulation  Purified mouse monoclonal in PB(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername  A26C1A;A26C1B;ACTB;ACTB_HUMAN;Actin cytoplasmic 1;Actin,beta;Actin,cytoplasmic 1,N-terminally processed;Actx;Beta actin;beta cytoskeletal actin;Beta-actin;E430023M04Rik;MGC128179;PS1TP5 binding protein 1;PS1TP5BP1.
Gene_symbol  BRWS1;PS1TP5BP1
Summary  This gene encodes one of six different actin proteins.Actins are highly conserved proteins that are involved in cell motility,structure,and integrity.This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.[provided by RefSeq,Jul 2008]
UniPort summary  Function  Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Application Images :  
Western blot detection of beta-actin antibody in C6,3T3,COS7,CHO-k1,K562 and Goat muscle cell lysates using beta actin antibody (1:10000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta-actin antibody in 5ug & 10ug S.cerevisiae and 5ug & 10ug P.pastoris cell lysates using beta actin antibody (1:5000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta-actin antibody in 15ug Arabidopsis and 30ug Arabidopsis cell lysates using beta actin antibody (1:1000diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta-actin antibody in Rabbit Spleen,Rabbit Colon,Rabbit Skeletal muscle,Rabbit Liver and Rabbit Heart cell lysates using beta actin antibody (1:5000 diluted).Predicted band size:45KDa.Observed band size:45KDa.